Morphological assessment of HEMA-MMA microcapsules for liver cell transplantation. by Julia Elizabeth Babensee

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Pagination312 leaves.
Number of Pages312
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Open LibraryOL18924530M
ISBN 100612281612

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Morphological assessment of hepatoma cells (HepG2) microencapsulated in a HEMA‐MMA copolymer with and without Matrigel Julia E. Babensee Department of Chemical Engineering and Applied Chemistry and Centre for Biomaterials, University of Toronto, Toronto, Ontario M5S 1A4 CanadaCited by:   Bioeng.39, Uludag, H.

and Sefton, M.V., Metabolic activity and proliferation of CHO cells in hydroxyethyl methacrylate- methyl methacrylate (HEMA-MMA) microcapsules, Cell Transplantation (in press) Babensee, J., De Boni, U. and Sefton, M.V., Morphological assessment ofhepatoma cells (HepG2) microencapsulated in a HEMA-MMA Cited by: Uludag, H.

and Sefton, M. () Metabolic activity and proliferation of CHO cells in hydroxyethyl methacrylate-methyl methacrylate (HEMA-MMA) microcapsules. Cell Transplantation 2, Cited by:   The Sefton group found that HEMA–MMA microcapsules were capable of postponing xenogeneic graft destruction, but not preventing it.

59 Having determined that the MWCO of their microcapsules was approximately kDa, 48 they postulated that shed antigens freely escape the microspheres and activate T cells. 59 The group implanted Cited by: The terpolymer of MAA-HEMA-MMA with or hereditary diseases of the liver, cell transplantation therapies can stimulate liver regeneration or serve as a bridge until liver transplantation can be.

The isolated cells can be used not only in research but also for transplantation in patients with liver disease, especially acute liver failure and liver-based metabolic/synthetic conditions.

Chenxia Hu, Lingfei Zhao, Zhongwen Wu, Lanjuan Li, Transplantation of mesenchymal stem cells and their derivatives effectively promotes liver regeneration to attenuate acetaminophen-induced liver injury, Stem Cell Research & Therapy, /s, 11, 1, ().

Transplantation of whole human pancreases or isolated islets into patients with type 1 diabetes mellitus has been severely hampered by the scarcity of cadaveric human donor organs, which mandates search for insulin producing cells/tissue source alternatives. Recent progress in stem cell biology has started looking into functionally competent, insulin-secreting progenitor cells.

Amin Vossoughi, Howard W. Matthew, Encapsulation of mesenchymal stem cells in glycosaminoglycans‐chitosan polyelectrolyte microcapsules using electrospraying technique: Investigating capsule morphology and cell viability, Bioengineering & Translational Medicine, /btm, 3, 3, (), ().

namely, primarily in hepatic parenchymal cells A rare autosomal dominant form of HH results from two categories of mutations in the gene for the iron transporter protein, ferroportin. ‘‘Loss-of-function’’ mutations decrease the cell surface localization of ferro-portin, thereby reducing its.

Experimental transplantation of mature hepatocytes and liver sinusoidal endothelial cells (LSECs) has improved the understanding of how hepatic and endothelial cell compartments could be.

We investigated the effect of bone marrow cell (BMC) transplantation on established liver fibrosis. BMCs of green fluorescent protein (GFP) mice were transplanted into 4‐week carbon tetrachloride (CCl 4)–treated C57BL6 mice through the tail vein, and the mice were treated for 4 more weeks with CCl 4 (total, 8 weeks).

Sirius red and GFP staining clearly indicated migrated BMCs. The effectiveness of cell transplantation can be improved by optimization of the transplantation site.

For some types of cells that form highly oxygen-demanding tissue, e.g., pancreatic islets, a successful engraftment depends on immediate and sufficient blood critical point can be avoided when cells are transplanted into a bioengineered pre-vascularized cavity which can be formed.

The Sefton group found that HEMA–MMA microcapsules were capable of postponing xenogeneic graft destruction, but not preventing it. 59 Having determined that the MWCO of their microcapsules was approximately kDa, 48 they postulated that shed antigens freely escape the microspheres and activate T cells.

59 The group implanted. Babensee JE, De Boni U, Sefton MV. Morphological assessment of hepatoma cells (HepG2) microencapsulated in a HEMA-MMA copolymer with and without Matrigel.

Biomed. Mater. Res. – PubMed CrossRef Google Scholar. Liver – Extramedullary Hematopoiesis Figure Legend: Figure 1 Extramedullary hematopoiesis–arrows indicate erythroid cells in a female Harlan Sprague-Dawley rat from a chronic study. Figure 2 Extramedullary hematopoiesis–arrow indicates erythroid cells, and arrowhead indicates a megakaryocyte, in a.

The feasibility of a microencapsulation process ultimately for cell transplantation was investigated by encapsulating human hepatoma (HepG2) cells in hydroxyethyl methacrylate–methyl methacrylate (HEMA–MMA) membranes through an interfacial precipitation process.

Changes in viability and metabolic activity as well as protein secretion by the encapsulated cells were studied in vitro. When. Liver diseases represent a major global health issue, and currently, liver transplantation is the only viable alternative to reduce mortality rates in patients with end-stage liver diseases.

However, scarcity of donor organs and risk of recidivism requiring a re-transplantation remain major obstacles. Hence, much hope has turned towards cell-based therapy. Hepatocyte-like cells obtained from. Objectives. Assessing the therapeutic efficacy of superparamagnetic iron oxide nanoparticles (SPIO) labeled bone marrow-derived mesenchymal stem cells (BM-MSCs) on experimental liver fibrosis and associated dysplasia.

Materials and Methods. MSCs were obtained from 10 male Sprague-Dawley rats while 50 female rats were divided into control (CG), liver fibrosis (CCL4, intraperitoneal injection. liver cells constitute an in vitro model for pharmacotoxicological studies and for the investigation of etiology and pathogenesis of human hepatocellular carcinoma." Homo sapiens adult CRL™ ZFL [ZF-L] liver Danio rerio adult HB™ AC Lymph node, liver.

Constituent Cells, Evidence for Cellular Compartmentalization, and Techniques for the Morphological Assessment of HEMA-MMA Microcapsules for Liver Cell Transplantation Michael V. Sefton Antonios G. Mikos, Rice Univ.,   The genipin cross-linked alginate-chitosan (GCAC) microcapsule, composed of an alginate core and a genipin cross-linked chitosan membrane, was recently proposed for live cell encapsulation and other delivery applications.

This article for the first time describes the details of the microcapsule membrane characterization using a noninvasive and in situ method without any physical. Transplantation Proceedings, [ Links ] Thivolet CH, Chatelain P, Nicoloso H, Durand A & Bertrand J ().

Morphology and function effects of extracellular matrix on pancreatic islets cell cultures. Experimental Cell Research, [ Links ]   Liver involvement by multiple myeloma presenting as 2Department of Medical and Morphological Sciences, University of Udine, University Hospital “Santa Maria della Misericordia”, Udine, and finally allogeneic stem cell transplantation from a matched, unrelated donor performed in.

Differentiation of encapsulated embryonic stem cells after transplantation. Transplantation. ; – Ding HF, Liu R, Li BG, Lou JR, Dai KR, Tang TT. Biologic effect and immunoisolating behavior of BMP-2 gene-transfected bone marrow-derived mesenchymal stem cells in APA microcapsules.

Biochem Biophys Res Commun. ; – At present, living cells are widely used in cell transplantation and tissue engineering. Many efforts have been made aiming towards the use of a large number of living cells with high activity and integrated functionality.

Currently, cryopreservation has become well-established and is effective for the long-term storage of cells. However, it is still a major challenge to inhibit cell damage. At a cell density of × 10 6 cells/cm 3 scaffold, the number of viable hepatocytes decreased to 33% of its initial value within 7 days, whereas at the denser cultures, × 10 6 cells/cm 3 scaffold and higher, the cells maintained higher viability while forming a network of connecting spheroids.

Sakai S, Hashimoto I, Tanaka S, Salmons B, Kawakami K. Small agarose microcapsules with cell-enclosing hollow core for cell therapy: transplantation of Ifosfamide-activating cells to the mice with preestablished subcutaneous tumor.

Cell Transplant. ; 18 (8)– Liver Transplantation 00 – AASLD. Citing Literature. Number of times cited according to CrossRef: 9. Byeong Geun Song, Dong Hyun Sinn, Wonseok Kang, Geum-Youn Gwak, Yong-Han Paik, Moon Seok Choi, Joon Hyeok Lee, Kwang Cheol Koh, Seung Woon Paik, Jong Man Kim, Jae-Won Joh, Gyu-Seong Choi, Changes in Tumor Markers and Their.

Wadei et al, Am J Transplantation, ; • ATN missed: renal support denied to patient • Need for simultaneous liver kidney transplant (SLK) • Incorrect assessment of treatments for HRS • HRS missed: vasoconstrictors not given.

This study examines cell junction components as hepatocytes form either monolayer or spheroid, and correlates morphological changes with cytochrome P function. Rat hepatocytes were cultured on either high-density (1 µ g/cm 2) or low-density (1 ng/cm 2) fibronectin, which promotes monolayers or spheroids, respectively.

Insulin-producing cells developed from embryonic stem cells may be an acceptable cell source for transplantation, and encapsulated cell or tissue implantation.

Cell Sourcing. Additional cell sources, organ specific progenitor cells from the brain, liver, and. Mesenchymal Stem Cells Combined with Hepatocyte Growth Factor Therapy for Attenuating Ischaemic Myocardial Fibrosis: Assessment using Multimodal Molecular Imaging. Sci. Rep. 6, ; doi: Biomechanical Assessment of Articular Cartilage Healing and Interspecies Variability Chemical Engineering and Applied Chemistry, Univ.

of Toronto, Morphological Assessment of HEMA-MMA Microcapsules for Liver Cell Transplantation: Michael V. Sefton: Antonios G. Mikos, Rice Univ., Chief of Division of Cellular. "Cultivation of fetal mouse liver cells in a three-dimensional poly-L-lactic acid scaffold in the presence of oncostatin M" Cell Transplant.,11(5), ().

Sakai, J. Jiang, N. Kojima, T. Kinoshita, and A. Miyajima "Enhanced in vitro maturation of fetal liver cells with oncostatin M, nicotinamide and dimethylsulfoxide". Investigation of a novel microcapsule membrane integrating polyethylene glycol to alginate, poly-l-lysine and chitosan microcapsules for the application of liver cell transplantation.

Abstract, XX International Congress of the Transplantation Society, SeptemberVienna. Plasma cell leukemia occurs when the number of plasma cells in the peripheral blood is 20% of the leukocyte differential count.

Other areas of involvement include spleen, liver, pleural effusions, ascites, and cerebrospinal fluid. PCL may be present at diagnosis or occur as a late feature of PCM (secondary PCL); % of myeloma cases are. OBM Transplantation is an international peer-reviewed Open Access journal, which covers all evidence-based scientific studies related to transplantation, including: transplantation procedures and the maintenance of transplanted tissues or organs; assimilation of grafted tissue and the reconstitution of removed organs or parts of organs; transplantation of heart, lung, kidney, liver, pancreatic.

Liver Cancer Cell of Origin, Molecular Class, and Effects on Patient Prognosis Daniela Sia,1 Augusto Villanueva,1 Scott L. Friedman,1 and Josep M. Llovet1,2,3 1Mount Sinai Liver Cancer Program, Divisions of Liver Diseases, Hematology, and Medical Oncology, Department of Medicine, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York; 2Liver Cancer Translational.

Hepatocellular carcinoma is one of the most common types of liver cancer causing death all over the world. Although early-stage liver cancer can sometimes be treated with partial hepatectomy, liver transplantation, ablation, and embolization, sorafenib treatment is the only approved systemic therapy for advanced HCC.

The aim of this research is to develop tools and methods to understand the. The liver plays a key role in both protein biosynthesis and lipid metabolism. As a result, hepatic synthetic dysfunction can have adverse effects on both cellular and soluble components of blood.

Anemia may occur due to the hemolysis of acanthocytes (spur cells), which is ultimately due to abnormal lipid composition of the red blood cell membrane.Mesenchymal stromal cells (MSCs) are able to migrate and engraft at sites of inflammation, injuries, and tumours, but little is known about their fate after local injection.

The purpose of this study is to perform MSC tracking, combining in vivo 7-T magnetic resonance imaging (MRI) and histological assessment, following lung injection in a rat model. Encapsulation of cells in microcapsules can prevent cell leakage (Zeng et al ), direct cells towards the desired phenotype (Hui et alRahman et alRui et al ) and protect the cells during the injection process (Wagner et al ).

Results from this study show that microcapsules remained viable after passing through a 25G needle.

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